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ORIGINAL ARTICLE
J Res Med Sci 2021,  26:75

Interleukin-6 and tumor necrosis factor-α levels in tear film of Keratoconus patients


1 Isfahan Eye Research Center, Department of Ophthalmology, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Biostatistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Date of Submission10-Jan-2021
Date of Decision05-Jun-2021
Date of Acceptance25-Jun-2021
Date of Web Publication30-Sep-2021

Correspondence Address:
Dr. Mohammad Namgar
Isfahan Eye Research Center, Department of Ophthalmology, Isfahan University of Medical Sciences, Isfahan
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jrms.jrms_35_21

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  Abstract 


Background: It is hypothesized that increased inflammatory markers in keratoconus (KC) may be one of the causes of corneal damage. The aim of our study was to the measurement of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL)-6 in tear of patients with KC and investigate their relationship with the severity of KC. Materials and Methods: The current study was performed on KC patients and healthy controls with a case-control setting. Tear levels of TNF-α and IL-6 were measured after collecting the tears from the tear lake using a filter paper via Schirmer I method without anesthesia. Results: Eighty-one KC patients (mean age 29.45 ± 5.06 years) and 85 controls (mean age 28.01 ± 5.14 years) were enrolled. The mean levels of IL-6 and TNF-α were 26.77 ± 8.16, and 34.58 ± 9.82 pg/ml in the healthy group and 103.22 ± 51.94, and 183.76 ± 54.61 pg/ml in the KC group, respectively (P < 0.001). There was a significant relationship between the severity of the KC and the mean levels of IL-6 TNF-α in the case group (P < 0.001). Conclusion: Our results indicated that the mean levels of IL-6 and TNF-α are significantly higher in KC than the healthy group, and the disease severity was significantly associated with TNF-α and IL-6.

Keywords: Inflammation, interleukin, keratoconus, tumor necrosis factor


How to cite this article:
Peyman A, Namgar M, Feizi A, Hakemi MG, Nasab FH, Pourazizi M. Interleukin-6 and tumor necrosis factor-α levels in tear film of Keratoconus patients. J Res Med Sci 2021;26:75

How to cite this URL:
Peyman A, Namgar M, Feizi A, Hakemi MG, Nasab FH, Pourazizi M. Interleukin-6 and tumor necrosis factor-α levels in tear film of Keratoconus patients. J Res Med Sci [serial online] 2021 [cited 2021 Dec 1];26:75. Available from: https://www.jmsjournal.net/text.asp?2021/26/1/75/327213




  Introduction Top


Keratoconus (KC) is characterized by progressive, noninflammatory stromal collagen changes and the central and paracentral corneal alternation.[1],[2] On one hand, there is an association of KC with atopic disease, allergies, and allergic rhinitis[3],[4],[5] and on the other hand, allergic conditions including atopic diastasis have considered as one of the important related factors associated with KC.[4],[5],[6]

The atopic situation may accelerate the course of KC[5],[7] because atopic conditions is usually associated with eye rubbings that is an important factor in KC and their progression.[8],[9]

KC has been considered a noninflammatory disease for many years, but recent investigations have shown inflammatory pathways in the mechanism of KC.[3],[6],[10],[11] Tear is an important and accessible source for the detection of biomarkers. Detection of some inflammatory cytokines including interleukin-1 (IL)-1 β, IL-6, interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α) in tear of patients with KC have been shown in several studies.[10],[12],[13]

In one study evaluating the amount of cytokines in tear of KC patients, researchers showed that cytokines such as matrix metallopeptidase 9 (MMP-9) and IL-6 are overexpressed in tears of KC patients and that the chronic inflammatory events in the eye may be the cause of KC progression.[14] Recently, there are clinical and experimental evidence that supports the role of proinflammatory cytokines in KC.[14],[15],[16]

The aim of the current study was to assess levels of TNF-α and IL-6 tears of patients with KC and healthy individuals and determine their relationship with disease severity.


  Materials and Methods Top


The current study was performed on patients with KC and healthy controls with normal corneal topography in a case-control design. The cases included KC patients (based on imaging findings) aged between 18 and 50 years. The control group was healthy age- and gender-matched individuals that were candidates for refractive surgery without any corneal diseases. Exclusion criteria were other corneal diseases (such as herpetic corneal inflammation and corneal opacity), pregnancy, breastfeeding, uveitis, and malignancy before or during the study. Written informed consent was obtained from each subject before the initiation of the study. The study was approved by the local ethical committee before the initiation (Project NO.: IR.MUI.MED.REC.1397.920).

Completed ophthalmological examination performed for all participants. One eye of each subject was randomly selected for inclusion in the study. Tears of the subjects were collected from the tear lake through the Schirmer I method via Schirmer tear test strip without anesthesia. The tear levels IL-6 and TNF-α were measured at baseline. The amount of tear obtained was calculated considering 1 mm of wet Schirmer strip containing 1 μL of tear. The levels of tear markers were measured through the highly sensitive method of enzyme-linked immunosorbent assay (ELISA). The limits of detection for the cytokine kits were 2 pg/ml.

Data were analyzed using SPSS (SPSS Inc., Chicago, Illinois, USA, Ver 21). Numerical variables were reported as mean ± standard deviation (SD) and median (mid-quartile range) while categorical variables were reported as frequency (percentage). The Spearman correlation coefficient and t-test were used to compare the two groups in terms of quantitative variables and disease severity. We used the Chi-square test to compare the two groups. Results were considered as statistically significant when P ≤ 0.05.


  Results Top


Eighty-one patients with KC and 85 healthy individuals were included study. The mean ± SD age of the patients was 29.45 ± 5.06 years in the control group and 28.01 ± 5.14 years in the case group [Table 1].
Table 1: Mean and frequency of demographic characteristics of individuals in the study groups

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The mean levels of IL-6 were 26.77 ± 8.16 pg/ml in the control group and 103.22 ± 51.94 pg/ml in the healthy controls and the mean levels of TNF-α were 34.58 ± 9.82 pg/ml in the control group and 183.76 ± 54.41 pg/ml in the case group. The effect of gender variable was considered constant using the mixed model analysis (by controlling the effect of gender variable) and the mean of IL-6, TNF-α, and keratometry of the individuals in the groups were examined. After modifying the amounts, there is significant differences between the groups regarding the level of IL-6 and TNF-α, and keratometry (P < 0.001) [Table 2].
Table 2: Mean levels of interleukin-6, tumor necrosis factor-a, and keratometry in the study groups

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A significant relationship existed between the disease severity (mean keratometry) and the levels of TNF-α and IL-6 in the case group (P < 0.001) [Table 3].
Table 3: Relationship of keratometry with interleukin-6 and tumor necrosis factor-a in the case group

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  Discussion Top


The current study indicated that the mean tear levels of TNF-α and IL-6 were significantly increased in KC patients compare to control groups. Mean keratometry in patients with KC was significantly associated with the levels of IL-6 and TNF-α.

Despite multiple studies conducted on the etiology of KC, its underlying cause still is unknown. It is proposed that increased inflammatory markers in patients with KC may be one of the causes of corneal damage and its progression.[10],[15],[17]

The presence of a vicious cycle between inflammatory cytokines and proteases and their inhibitors, as well as excessive oxidative stress, result in increased apoptosis.[14],[18],[19]

The results of our study showed a significant increase of IL-6 and TNF-α and the disease severity (mean keratometry) in tears of KC. In this regard, a study was performed aiming to determine the levels of IL-4, IL-6, IL-10, RANTES, INF-γ, and TNF-α in tears of 48 patients with KC and their first-degree family members. The researchers showed that the levels of inflammatory markers such as INF-γ, IL-10, IL-1 β, IL-4, IL-6, and TNF-α significantly increased in patients with KC and their first-degree family members compared to the control group.[12]

Sorkhabi et al. determined the level of inflammatory markers in tear of 42 KC patients and 30 controls. Similar to our study, Sorkhabi et al. demonstrated that there is a statistically significant difference in the levels of inflammatory markers such as IL-6, IL-10, IL-1 β, and IFN-γ in tears of patients with KC versus control group.[16]

While in a review study aimed at answering the question “is KC an inflammatory disorder?” Galvis et al. demonstrated that the increased levels of IL-6, TNF-α, and MMP-9 in the lacrimal fluid of KC patients do not meet all the classical criteria of inflammatory disease.[14] This can be attributed to the limited number of studies performed by one kit, while the majority of them use the standard ELISA with less sensitivity to multiplex polymerase chain reaction. In their review study, Ionescu et al. suggested that there are some hypotheses about potential and different pathways in the expression of inflammatory markers in patients with KC.[15]

Although overexpression of cytokines may be a cause of inflammation in KC and overexpression of inflammatory biomarkers may be a complementary risk factor, the present study showed a significant relationship between disease severity and IL-6 and TNF-α expression in the KC group. Similarly, Ionescu et al. found a statistically significant relationship between lacrimal expression of IL-6 and disease severity in the KC group.[12]

Similar to our finding, Balasubramanian et al. reported that the expression of IL-4, IL-5, TNF-α, IL-10, and IL-6 increased in the tears of patients with KC. Therefore, KC can be classified as inflammatory conditions. Moreover, there was a positive correlation between cytokines and keratometry.[20]

Although our study had several limitations, larger sample size compared to other studies was the strength of the study. Results of our study provide promising horizons about the feasibility of inflammatory response modulators in the control of the progression of disease. Our study had some limitations. We have evaluated only two main proinflammatory cytokines, IL-6 and TNF-a in the tears of patients with KC. Another limitation was the lack of evaluating the level of these cytokines in blood samples for assessment of systemic involvement in KC disease.


  Conclusion Top


The current study showed that the levels of IL-6 and TNF-α increased in KC patients compared to healthy individuals. Furthermore, change of level of IL-6 and TNF-α significantly associated with increasing disease severity. Biochemical findings might help diagnose and determine the severity and progression of KC.

Availability of data and materials

All datasets related to the current study are available from the corresponding author.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Zhang X, Munir SZ, Sami Karim SA, Munir WM. A review of imaging modalities for detecting early keratoconus. Eye (Lond) 2021;35:173-87.  Back to cited text no. 1
    
2.
Henriquez MA, Hadid M, Izquierdo L Jr., A systematic review of subclinical keratoconus and forme fruste keratoconus. J Refract Surg 2020;36:270-9.  Back to cited text no. 2
    
3.
McKay TB, Serjersen H, Hjortdal J, Zieske JD, Karamichos D. Characterization of tear immunoglobulins in a small-cohort of keratoconus patients. Sci Rep 2020;10:9426.  Back to cited text no. 3
    
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Ahuja P, Dadachanji Z, Shetty R, Nagarajan SA, Khamar P, Sethu S, et al. Relevance of IgE, allergy and eye rubbing in the pathogenesis and management of Keratoconus. Indian J Ophthalmol 2020;68:2067-74.  Back to cited text no. 4
[PUBMED]  [Full text]  
5.
Galvis V, Tello A, Carreño NI, Berrospi RD, Niño CA. Risk factors for keratoconus: Atopy and eye rubbing. Cornea 2017;36:e1.  Back to cited text no. 5
    
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Shetty R, Sureka S, Kusumgar P, Sethu S, Sainani K. Allergen-specific exposure associated with high immunoglobulin E and eye rubbing predisposes to progression of keratoconus. Indian J Ophthalmol 2017;65:399-402.  Back to cited text no. 6
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Woodward MA, Blachley TS, Stein JD. The association between sociodemographic factors, common systemic diseases, and keratoconus: An analysis of a nationwide heath care claims database. Ophthalmology 2016;123:457- 65.e2.  Back to cited text no. 7
    
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Peyman A, Feizi A, Ganjalikhani-Hakemi M, Hosseini-Nasab F, Pourazizi M. Outcome of corneal collagen cross-linking in keratoconus: Introducing the predictive factors. J Curr Ophthalmol 2020;32:19-25.  Back to cited text no. 8
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Najmi H, Mobarki Y, Mania K, Altowairqi B, Basehi M, Mahfouz MS, et al. The correlation between keratoconus and eye rubbing: A review. Int J Ophthalmol 2019;12:1775-81.  Back to cited text no. 9
    
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Wisse RP, Kuiper JJ, Gans R, Imhof S, Radstake TR, Van der Lelij A. Cytokine expression in keratoconus and its corneal microenvironment: A systematic review. Ocul Surf 2015;13:272-83.  Back to cited text no. 10
    
11.
Acera A, Vecino E, Rodríguez-Agirretxe I, Aloria K, Arizmendi JM, Morales C, et al. Changes in tear protein profile in keratoconus disease. Eye (Lond) 2011;25:1225-33.  Back to cited text no. 11
    
12.
Ionescu IC, Corbu CG, Tanase C, Ionita G, Nicula C, Coviltir V, et al. Overexpression of tear inflammatory cytokines as additional finding in keratoconus patients and their first degree family members. Mediators Inflamm 2018;2018:4285268.  Back to cited text no. 12
    
13.
Jun AS, Cope L, Speck C, Feng X, Lee S, Meng H, et al. Subnormal cytokine profile in the tear fluid of keratoconus patients. PLoS One 2011;6:e16437.  Back to cited text no. 13
    
14.
Galvis V, Sherwin T, Tello A, Merayo J, Barrera R, Acera A. Keratoconus: An inflammatory disorder? Eye (Lond) 2015;29:843-59.  Back to cited text no. 14
    
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Ionescu C, Corbu CG, Tanase C, Jonescu-Cuypers C, Nicula C, Dascalescu D, et al. Inflammatory biomarkers profile as microenvironmental expression in keratoconus. Dis Markers 2016;2016:1243819.  Back to cited text no. 15
    
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Sorkhabi R, Ghorbanihaghjo A, Taheri N, Ahoor MH. Tear film inflammatory mediators in patients with keratoconus. Int Ophthalmol 2015;35:467-72.  Back to cited text no. 16
    
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Shetty R, Sharma A, Pahuja N, Chevour P, Padmajan N, Dhamodaran K, et al. Oxidative stress induces dysregulated autophagy in corneal epithelium of keratoconus patients. PLoS One 2017;12:e0184628.  Back to cited text no. 17
    
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Sharif R, Bak-Nielsen S, Hjortdal J, Karamichos D. Pathogenesis of keratoconus: The intriguing therapeutic potential of prolactin-inducible protein. Prog Retin Eye Res 2018;67:150-67.  Back to cited text no. 18
    
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Khaled ML, Helwa I, Drewry M, Seremwe M, Estes A, Liu Y. Molecular and histopathological changes associated with keratoconus. Biomed Res Int 2017;2017:7803029.  Back to cited text no. 19
    
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Balasubramanian SA, Mohan S, Pye DC, Willcox MD. Proteases, proteolysis and inflammatory molecules in the tears of people with keratoconus. Acta Ophthalmol 2012;90:e303-9.  Back to cited text no. 20
    



 
 
    Tables

  [Table 1], [Table 2], [Table 3]



 

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