Postoperative outcomes of intrasphincteric botox injection during hemorrhoidectomy: A double-blind clinical trial
Mina Alvandipour1, Mehdi Tavallaei2, Fatemeh Rezaei3, Hamed Khodabakhsh4
1 Department of Surgery, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Mazandaran Province, Iran
2 Department of Surgery, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Medical Surgical Nursing, School of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran
4 Department of Emergency Medicine, Mazandaran University of Medical Sciences, Sari, Iran
|Date of Submission||03-Aug-2018|
|Date of Decision||15-Aug-2018|
|Date of Acceptance||30-Dec-2020|
|Date of Web Publication||30-Aug-2021|
Dr. Mehdi Tavallaei
Department of Surgery, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan
Source of Support: None, Conflict of Interest: None
Background: Pain is the most common postoperative complication of hemorrhoidectomy. We evaluated the effectiveness of intrasphincteric Botox injection on posthemorrhoidectomy complications including pain reduction and wound healing. Materials and Methods: In this randomized, double-blind clinical trial, patients with Grades 3 or 4 symptomatic hemorrhoids who underwent open (Milligan-Morgan) hemorrhoidectomy were enrolled. The experimental group received intrasphincteric Botox injection during hemorrhoidectomy, while the controls received normal saline injection. Hemorrhoid grades, constipation status, history of hemorrhoidectomy, duration of operation, pain at rest and after defecation in six follow-up periods (6, 12, 24, and 48 h and 7 and 14 days after operation), wound healing (during follow-up after discharge with a 2-week period), analgesic use, and Botox side effects were evaluated and compared in the two experimental and control groups. Results: In this trial, 34 and 33 patients were randomly allocated in the experimental and control groups, respectively. Operation time was significantly higher in Botox group (P = 0.009). Mean dose of analgesics use in Botox was significantly lower (P < 0.001). Rate of wound healing during follow-ups was significantly higher in Botox group in the fifth follow-up (P = 0.009). Frequency of urinary retention (P = 0.02) and moderate itching (P = 0.01) was significantly higher in placebo than Botox group. Mean of postoperative pain at rest in Botox group was significantly lower at 12th, 24th, and 48th h and 7th and 14th days after operation (P < 0.01). Mean of postoperative pain in Botox group was significantly lower at 3rd to 5th defecation (P < 0.01). Conclusion: Our findings indicated that a single-dose injection of Botox during Milligan-Morgan hemorrhoidectomy is associated with less postsurgical pain at rest and during defecation and improved wound healing. It is suggested that it is a safe and effective procedure during hemorrhoidectomy regarding the procedure-related complications.
Keywords: Botox, Botulinum toxin, hemorrhoidectomy, pain
|How to cite this article:|
Alvandipour M, Tavallaei M, Rezaei F, Khodabakhsh H. Postoperative outcomes of intrasphincteric botox injection during hemorrhoidectomy: A double-blind clinical trial. J Res Med Sci 2021;26:53
|How to cite this URL:|
Alvandipour M, Tavallaei M, Rezaei F, Khodabakhsh H. Postoperative outcomes of intrasphincteric botox injection during hemorrhoidectomy: A double-blind clinical trial. J Res Med Sci [serial online] 2021 [cited 2021 Dec 3];26:53. Available from: https://www.jmsjournal.net/text.asp?2021/26/1/53/325064
| Introduction|| |
Hemorrhoids represent one of the most common disorders in the anorectal region. At least 37% of people aged over 37 years suffer from hemorrhoids. Although hemorrhoidectomy is considered a minor surgery, it is accompanied by postoperative complications such as pain, bleeding, feeling of heaviness, itching, mucus secretions, and anal stenosis. These complications can have many causes including individual tolerance, type of anesthesia, use of postoperative analgesics, and surgical techniques.
Pain is the most common postoperative complication of hemorrhoidectomy, and thus one of the most important considerations of this surgery is pain control. If pain is controlled, it can allow for an earlier return to work and reduce urinary retention, unsuccessful fecal discharge, and hospitalization costs.
Discomfort in the anoderm and spasms in the internal sphincter muscles are the two main underlying reasons for posthemorrhoidectomy pain.
Various surgical and nonsurgical methods have been investigated in order to control pain. Treatments using metronidazole, steroids, nonsteroidal anti-inflammatory drugs, topical anesthetics, and opium have been used and produce different effects; however, since the effects are short term and accompanied by complications; thus, they have limited value.
Some surgical procedures may reduce anal spasms after hemorrhoidectomy more effectively than others; conversely, some studies have reported that surgical procedure has little impact on pain relief. Moreover, some studies have reported that incontinency can result from surgery.,,
Evidences indicated that botulinum neurotoxin which is considered as a multipurpose therapeutic agent could be used in several clinical applications in modern medicine. One of the most usage of the neurotoxin is its usage in pain management.
The role of botulinum neurotoxin in the inhibition of pain receptors has been previously explained. In addition, the effects of Botox on pain relief in other conditions such as adductor muscle release in patients with cerebral palsy, and in patients with neck dystonia, chronic migraine, piriformis syndrome, chronic epicondylitis, mastectomy, and patients after hernia repair and cholecystectomy have also been evaluated.,, There were few studies regarding the effectiveness of Botox injection in pain reduction and improving wound healing after hemorrhoidectomy.,
Although different clinical uses of Botox have been reported in previous studies there were limited studies regarding the usefulness of Botox in hemorrhoidectomy. It is suggested that Botox could be a proper analgesic agent for reducing hemorrhoidectomy-related pain and complications due to its some characteristics such as high efficacy, longevity of action, and satisfactory safety profile. So that, we aimed to assess the effects of intrasphincteric Botox injection on posthemorrhoidectomy complications, wound healing, and pain reduction.
| Materials and Methods|| |
This was a prospective, double-blinded clinical trial conducted at Razi Hospital in Ghaem-Shar and Imam Khomeini Hospital in Sari from March 2015 to March 2016. This study was approved by regional ethics committee of Mazandaran University of Medical Sciences with a research project number of IR.MAZUMS.REC.95.1836. Seventy patients aged more than 18 years, with 3rd or 4th grades of symptomatic hemorrhoids who indicated for hemorrhoidectomy were recruited to the trial. Patients with inflammatory bowel disease, fistula, fissure, and a history of previous surgery in the anal region, dermatitis, proctitis, pregnancy, and cardiovascular or severe respiratory diseases were excluded. Written informed consent was obtained from the selected patients. Selected patients were randomly allocated to either the Botox or placebo group using table of random numbers. The experimental group received intrasphincteric injections of Botox and the placebo group received normal saline using the same method.
All patients underwent routine clinical assessments including digital rectal examination and proctoscopy. Colonoscopy or sonography was performed according to the recommendation of colorectal surgeon. All routine laboratory tests were performed.
Both surgeon and patients were unaware of the group to which they were assigned.
Although the study was conducted in two centers, all surgeries were performed out by one surgeon (MA) using the same anesthesia protocol in order to reduce any interpersonal variation.
None of the patients received prophylactic antibiotics. They were sedated with intravenous fentanyl and propofol. Patients were placed in the lithotomy position and underwent electrocautery open hemorrhoidectomy using the Milligan-Morgan technique by a subspecialist colorectal surgeon.
Botulinum toxin A was diluted in saline (50 units/mL). The Botox solution (0.4 mL; 20 units) was divided into two 0.2 mL doses and injected with a 25G syringe into either side of the anterior midline of the internal sphincter. The placebo group received 0.4 mL of normal saline with a 25G syringe injected into the same regions as other groups. Perioperative management was similar for all patients. Patients received Apotel ampoules 1 g every 6 h IV infusion and patients who had a pain score equal to/above 6 (based on a visual analog scale [VAS]) received intramuscular injections of 0.5 mg/kg morphine sulfate.
A surgical resident, who was blinded to the results of randomization, evaluated the patients before, during, and after surgery. Before surgery, he recorded the baseline characteristics of patients including sex, constipation status before the operation, and history of hemorrhoidectomy. During surgery, the investigator recorded the duration of operation and dose of analgesics used in first 24 h. After operation, the resident evaluated and recorded postoperative complications including bleeding, incontinence, anal stenosis, urinary retention, anal fullness, itching, and amount of secretions.
The primary outcome measures were pain intensity at rest and during defecation which scored based on the VAS by the surgical resident, at 6 and 12 h after surgery and again on days 1, 2, 7, and 14. Pain from the first to fifth defecation was also evaluated.
The secondary outcome measure was wound healing which was evaluated during follow-ups. Wound healing was assessed in terms of incidence of wound secretions, type of wound discharge, and itching. Five follow-up examinations were performed from day 30 to 4 months after the surgery (follow-up interval was a 2-week period).
The investigator recorded Botox-related complications including perianal thrombosis, subcutaneous abscess, hematoma, perianal itching, and urinary stress incontinence.
Three patients (one from the Botox group and two from the placebo group) were excluded because they did not participate in postsurgical follow-ups.
Data were analyzed using Statistical Package for Social Sciences (SPSS Inc., version 21.0, Chicago, IL, USA). Continuous variables were presented as mean (standard deviation). Categorical variables were presented as number (%). Continuous variables between groups were compared using Student's t-test and categorical data were compared using Chi-square and Fisher's exact tests. P < 0.05 was considered statistically significant between the two groups.
| Results|| |
In this trial from initially allocated seventy patients, three discontinued the follow-ups and finally 67 patients completed the study [Figure 1].
Baseline characteristics of studied patients in the intervention and control groups are presented in [Table 1]. Two studied groups were similar regarding sex and baseline characteristics.
|Table 1: Baseline characteristics of studied patients in Botox and placebo groups|
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Operative data (mean duration of operation, mean doses of analgesics in the first 24 h) and postoperative outcomes including frequency of bleeding, gas incontinence, anal stenosis, urinary retention, itching severity, type of wound secretion, and rate of wound healing during follow-ups are presented in [Table 2].
|Table 2: Operation related variables and postoperative outcomes of studied patients in Botox and placebo groups|
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Mean duration of operation and mean dose of analgesics use in first 24 h in Botox was significantly higher (P = 0.009) and lower (P < 0.001) than placebo group, respectively.
From studied postoperative outcomes, frequency of urinary retention (P = 0.02) and moderate itching (P = 0.01) was significantly higher in placebo than Botox group.
Rate of wound healing (it presented with %) during follow-ups was significantly higher in Botox group than placebo group in the fifth follow-up (P = 0.009).
Mean of pain experienced at rest and during defecation at different posthemorrhoidectomy follow-ups based on VAS is presented in [Figure 2] and [Figure 3].
|Figure 2: Mean of pain experienced at rest at different posthemorrhoidectomy follow-ups based on visual analog scale in Botox and placebo groups|
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|Figure 3: Mean of pain experienced during defecation at different posthemorrhoidectomy follow-ups based on visual analog scale in Botox and placebo groups|
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Mean of postoperative pain at rest in Botox group was significantly lower at 12th, 24th, and 48th h and 7th and 14th days after operation (P < 0.01).
Mean of postoperative pain during defecation in Botox group was significantly lower during 3rd to 5th defecation than placebo group (P < 0.01).
None of the patients who received Botox complained of adverse effects from this treatment.
| Discussion|| |
In this clinical trial, we evaluated the effectiveness of intrasphincteric Botox injections on outcomes of hemorrhoidectomy. Our findings indicated that though by using intrasphincteric Botox injections during hemorrhoidectomy, the operation time was longer, but the frequency of some postoperative complications including urinary retention and itching was lower in Botox group. The most important and favorable outcome of intrasphincteric Botox injection was reduction of postoperative pain at rest and during defecation and better wound healing at fifth follow-up.
Internal sphincterotomy is one of the procedures used to reduce maximum resting anal pressure (MRP), and it can reduce the number of posthemorrhoidectomy complications. The use of drugs to reduce MRP has increased following the recent introduction of chemical sphincterotomy methods. Studies on the effects of these methods on postsurgical pain – especially after defecation – are rare and controversial. The use of drugs such as glycerin nitrate to reduce MRP is associated with lower pain at rest but can cause headaches and vertigo, which are not well tolerated by patients., One nonrandomized prospective study showed that nitroglycerin caused a reduction in pain score, reduced the need for analgesics, and enabled an earlier return to normal physiological state in patients with high MRP but did not affect those with normal MRP before surgery.
Although botulinum neurotoxin or Botox is considered as a powerful therapeutic agent which is recently used in different medical conditions, there are few studies related to its effectiveness in reducing postoperative pain and wound healing after hemorrhoidectomy.
Botox inhibits acetylcholine release in presynaptic nerve terminals and subsequent noradrenaline release from sympathetic neural terminals, which causes transient paralysis of the internal sphincter. However, this drug does not entirely eliminate voluntary sphincter control. Weakness occurs within hours but impulse transmission will recommence following re-growth of new neuromuscular junctions within 3–4 months.
Botulinum toxin has numerous potential advantages over other methods for the reduction of internal sphincter pressure including single-dose treatment, lack of complications, lower costs and, in time, greater perineal sensitivity of the internal sphincter.
In this study, we used the Milligan-Morgan hemorrhoidectomy technique. Numerous prospective trials have compared this method with stapledhemorrhoidectomy and found that the stapledhemorrhoidectomy group experienced less pain.,, However, there are some concerns about the prognosis of the stapled technique including painful defecation and incontinence, tearing of the internal sphincter, and costs. Although the Milligan-Morgan method is still considered as the gold standard method for hemorrhoidectomy, currently, postoperative pain remains the major problem of the method. So that, the necessity of studying the effectiveness of different agents is considered a priority in this field for surgeons. In current trial, we evaluated the role of Botox in this field.
In literature review, there were three randomized controlled trials which investigated the postoperatives outcomes of Botox after Milligan–Morgan hemorrhoidectomy.,,
The first study was conducted by Davies et al. in the UK. They studied 49 patients in Botox and normal saline groups. Pain was scored using VAS. They indicated that using Botox resulted in less pain on day 6 and 7 after surgery. They suggested that pain reduction may be due to the decreased spasm within the internal sphincter. They did not report significant difference in analgesic use between two studied groups.
Patti et al. in Italy, in two studies investigated the effectiveness of Botox in posthemorrhoidectomy pain and wound healing. They indicated less pain both at rest and during defecation a week after surgery and less analgesic use as well as proper wound healing without any complication or adverse effects.,
Singh et al. in the UK, in a prospective randomized controlled trial among 32 patients with Grade 3 and 4 hemorrhoid, demonstrated that Botox did not reduce postoperative pain but it could reduce anal spasm.
Siddiqui et al. in a review study evaluated the effectiveness of different agents used for chemical sphincterotomy after Milligan–Morgan hemorrhoidectomy. They concluded that Botox is one of the agents which could provide proper postoperative pain relief after Milligan–Morgan hemorrhoidectomy.
In the current study, operation time was significantly higher in Botox group, but the outcomes were more favorable.
In the current study, mean dose of analgesic use in the first 24 h was lower in the Botox group. Davies et al. reported no difference between the groups. Our finding in this regard was similar to Patti et al.
In this study, some complications such as urinary retention and itching were less frequent in Botox group. Previous studies reported that these complications in Botox groups appear comparable to placebo.
In our study, wound healing was evaluated for 4 months after hemorrhoidectomy. The results showed that the process of wound healing was more advanced in the Botox group than in the placebo group at the end of 4th month (75% in comparison to 85%). Patti et al. showed earlier wound healing in Botox group. In another study, Patti et al. compared Botox and glycerin nitrate and found no difference in wound healing between the two groups. It seems that quicker wound healing is considered an important outcome because consequently it is associated with earlier return to work or normal activity.
The primary outcome of our study was evaluation of pain at rest and during defecation. Our results were similar to that reported by Patti et al. and Davies et al., Singh et al. did not find any significant difference between groups. It could be explained by that they used local anesthetic at the end of the operation. However, differences in mentioned studies with our may be due to heterogeneity of the trials such as type of botulinum toxin (BOTOX or DYSPORT), using antibiotics, laxative, or other agents during surgery.
The main limitation of the current study was that we did not use anal manometry on patients before or after surgery.
The strength of our study was its design as double-blind prospective clinical trial, larger sample size in comparison with previous studies. Moreover, we compared the outcomes in longer duration.
| Conclusion|| |
A single-dose injection of botulinum toxin into the internal anal sphincter during Milligan-Morgan hemorrhoidectomy was associated with less postsurgical pain at rest and during defecation and improved wound healing. Botox injection was also more efficient and had fewer complications. Therefore, it is suggested that it is a safe and effective procedure during hemorrhoidectomy regarding the procedure-related complications.
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Conflicts of interest
There are no conflicts of interest.
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[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2]