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ORIGINAL ARTICLE
Year : 2020  |  Volume : 25  |  Issue : 1  |  Page : 111

The significant suppressive effect of intravenous injection of autologous bon marrow-derived mesenchymal stem cells on the gene expression and plasma level of CCL5 in patients with refractory rheumatoid arthritis


1 Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2 Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3 Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Internal Medicine Department, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
4 Student Research Committee, School of Medicine, Bam University of Medical Sciences, Bam, Iran; School of Medicine, Bam University of Medical Sciences, Bam, Iran
5 Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran; Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
6 Department of Pediatric, Hematology and Oncology and Stem cell Transplantation, Mashhad University of Medical Sciences, Mashhad, Iran
7 Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence Address:
Dr. Mojgan Mohammadi
Allergy Research Center, Mashhad University of Medical Sciences, Mashhad
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jrms.JRMS_308_20

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Background: Rheumatoid arthritis (RA) is the most prevalent autoimmune disease, in which CCL2 and CCL5 are critically involved. The objective of this study was to evaluate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) on the foregoing chemokines in RA patients. Materials and Methods: Thirteen RA patients were evaluated in terms of clinical manifestations, paraclinical factors, gene expression, and plasma levels of CCL2 and CCL5 prior to treatment and 1 and 6 months after intervention. Real-time-polymerase chain reaction and enzyme-linked immunosorbent assay were employed to assess the gene expression and plasma levels of CCL2 and CCL5 at different time points after MSC therapy. Statistical analysis was performed by SPSS 16 and Prism 7. Results: The CCL2 gene expression had significantly increased (P = 0.034), and its plasma level had insignificantly reduced after 1 month. Furthermore, the gene expression and plasma level of CCL5 had statistically significantly decreased (P = 0.032, P < 0.001). The CCL5 gene expression had statistically increased after 6 months (P = 0.001) and its plasma level had insignificantly reduced. Conclusion: The most significant inhibitory effects of MSC therapy on the gene expression and plasma level of CCL5 were observed at the end of 1 month. The differences between the gene expression and protein levels during the treatment might be related to microRNA effects or the insufficient number of MSC injection.


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